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1 mL 20% TCA followed by 1 mL 10% TCA solutions were added to the centrifuged (10,000 g 10 min at 4 C) pellet after discarding the supernatant

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FIGURE 3 FIGURE 4 FIGURE 5 FIGURE 6 When -glutamyl- S -allyl-L-cysteine sulfoxide was used as a -glutamyl donor substrate, only small amounts of alliin were detected in assays using crude protein extracts from yeast expressing AsGGT1 or AsGGT3 ( Figure 4

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