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Samples were analyzed by 12% urea-PAGE run on an Apogee S2 sequencing gel apparatus at 1200 V for 6 h

[DOI] [PMC free article] [PubMed] [Google Scholar] 1442.Zhang YJ, Xu YF, Liu YH, Yin J, Li HL, Wang Q, Wang JZ

One example of a prodrug is levodopa, a prodrug of dopamine used for the treatment of Parkinsons disease

We attempted to antagonise this effect on stability by further increasing the net negative charge of scFvs at a lower pH and by decreasing their pI through fusion of the human influenza hemagglutinin (HA) tag (YPYDVPDYA) with low pI (3.53) and a net negative charge (2.2) to the C-terminus of s3Flag-scFv-A36 or s3Flag-scFv-M4 (Fig

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