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Thus, studies on the combination of strategies that can penetrate a normal BBB or on the dynamics of BBB disruption, as well as on the identification of biomarkers of brain vascular injury, might pave the way to new drug delivery approaches targeting BBB in the near future

In situ hybridization In situ hybridization (ISH) was performed on fresh frozen coronal brain sections (25 m) according to protocols that are available at Briefly, digoxigenin (DIG)-labeled RNA probes were transcribed in vitro from cloned cDNAs for mouse Pdgfra, Enpp6, Plp, Mbp and Mog as previously described (Huang et al., 2018

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