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Folcik VA, Garofalo M, Coleman J, Donegan JJ, Rabbani E, Suster S, Nuovo A, Magro CM, Di Leva G, Nuovo GJ (2013) Idiopathic pulmonary fibrosis is strongly associated with productive infection by herpesvirus saimiri

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the reduced dye then undergoes non-enzymic oxidation in air, so the addition of a relatively small amount of methylene blue will effectively deplete NADPH, and would be expected to stimulate any pathway that reduces NADP+ to NADPH

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The resulting formazan crystals were dissolved in 100 L of dimethyl sulfoxide (DMSO) with gentle shaking for 5 min, and absorbance was subsequently measured at 570 nm using a microplate reader