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Likewise, assays of GCL and GS activity in the cytosol and chloroplast fractions of Arabidopsis leaf tissue show specific activities as follows: GCL cytosol 2.2 0.3 nmol min 1 mg total protein 1 (18% total activity) and GCL chloroplast 10.0 0.6 nmol min 1 mg total protein 1 (82% total activity)

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doi:10.1016/j.jmb.2005.05.014 ^ a b c Mattevi A., Obmolova G., Sokatch J.R., Betzel C., Hol W.G

This approach has been already tested in canine models for the treatment of Duchenne Muscular Dystrophy (Amoasii et al., 2018), and despite it has not been tested in animal models for the treatment of CF, it has been found effective in correcting CFTR defects (Hsu et al., 2014)

Filtered viral supernatant was then concentrated by Lenti-X Concentrator (#631232, Takara) following the manufacturers instruction and the virus pellet was resuspended in 1/100th of the original volume using cold DMEM

doi:10.1016/0014-5793(90)80922-6