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The IL-1, IL-6, and TNF- concentrations in the liver tissue were measured using commercial rat ELISA quantification kits according to the instructions of the manufacturer

doi:10.1016/j.physbeh.2018.01.006

However, unlike in the gourami, mild intermittent nocturnal hypoxia (17 kPa PO 2 ) actually accelerated (brought forward) the onset of air breathing by 4 days and as this species progressively became a facultative air-breather, ultimately being able to extract oxygen from both air and water, an example of developmental flexibility

In the body it is formed from the three amino acids glutamine acid, cysteine and glycine in a two-stage synthesis process

Bolitho, E

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