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Media was discarded from the plate then, 50 l of different concentrations of CuNPs or nano-chrysin (0.5, 1.0, 2.0, 3.9, 7.8, 15.6, 31.25, 62.5, 125, 250, 500, and 1000 g/ml) and 50 l of MTT solution were added into each well and incubated for 3 h at 37 C

LC-MS/MS analysis of GS-TPP and GSS-TPP MS1 and product ion scans were performed by direct infusions into a Xevo TQ-S triple quadrupole mass spectrometer (Waters, UK)

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