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Captured mAb variants were detected by an APconjugated polyclonal anti-human IgG Fc specific Ab from goat (Sigma-Aldrich, A9544) (diluted 1:5000 in PBS/T/S) and visualized by addition of p-nitrophenylphosphate (Sigma-Aldrich) (10 g/mL in diethanolamine buffer)

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We demonstrate that PSR-1 forms condensates in vitro and in vivo and that the biophysical properties of PSR-1 condensates can be altered by hydrogen peroxide and DTT