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Mathew SJ, Mao X, Coplan JD, Smith ELP, Sackeim HA, Gorman JM, et al

Interference in the development of a secondary immune response in mice by zinc deprivation: persistence of effects

NHS (15% for the AP, 1% for the CP, and 2% for the LP) was then pre-incubated with a final concentration of 4 M (initial analyses) or increasing concentrations (0.5, 1, and 4 M) (dose dependence analyses) of purified His 6 -tagged proteins for 15 min at RT before being added to the wells to initiate complement activation of the respective pathway

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This led to elegant work on StAR action involving its molecular tether to the OMM, IMS, and IMM which indicated that StAR acts on the OMM and that mitochondrial import to the matrix inactivates StAR activity (139)