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Methods Enzymol 186:421431 Duan W, Lee J, Guo Z, Mattson MP (2001) Dietary restriction stimulates BDNF production in the brain and thereby protects neurons against excitotoxic injury

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Primary antibodies used for the recognition of OL antigens were MBP (1:200) and Olig2 antibody (1:200), followed by goat anti-rat IgG Alexa Fluor or goat anti-rabbit Alexa Fluor (see antibody characterization below)

Most anti-neoplastic drugs, which are often hydrophilic and large, cannot cross the BBB efficiently due to their size and polarity (87, 88)

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Also, the framework assumes steady-state metabolism and constant photon input, thereby limiting its ability to represent dynamic temporal metabolic reprogramming