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Phosphate buffer saline (PBS), Dulbeccos modified Eagles medium (DMEM), RPMI-1640 with L-glutamine medium (RPMI-1640), fetal bovine serum (FBS), penicillin/streptomycin and trypsin-EDTA 0.25% were purchased from Hyclone (Waltham, MA, USA)

Briefly, 1 10 6 primary CD4 + T cells were lysed in 133 l of lysis buffer 10 mM TrisHCL, 50 nM KCl, and 400 g/ml proteinase K