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The main fermentation was conducted in 100 mL of YCK50D medium (Cha et al., 2004) containing 30 g/L yeast extract, 5 mM cysteine, 0.6 g/L KH 2 PO 4 , and 50 g/L glucose

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10.1002/jcp.31421 22

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Keywords: Glutathione reductase inhibitor, thiol redox state, protein thiols, GSH, GSSG Glutathione reductase (GR) is a homodimeric flavoprotein of 252 kDa [1] and an important antioxidant enzyme in maintaining an intracellular reducing environment, which is critical to the cell against oxidative stress