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2.10 Overexpression and knockdown of RRM2 gene in TPC-1 and IHH4 cells For upregulating RRM2 expression in cells, we obtained a full-length RRM2 sequence fragment using a PCR method and subsequentlycloned it into vector LV18, verified by DNA sequencing and transfected 293T cells (see Supplementary Materials for details)

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Role of GST P1-1 in mediating the effect of etoposide on human neuroblastoma cell line Sh-Sy5y

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Finally, the lack of regulatory oversight in many countries regarding dietary supplements compared to pharmaceutical drugs increases the risk of contamination or mislabeling 64 , further highlighting the importance of selecting supplements from reputable manufacturers