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Most human trials use the full 43-amino-acid thymosin beta-4 (RGN-259, RGN-137), not the TB-500 fragment

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The peptide mixture was collected by centrifugation and acidified with 10% (v/v) trifluoroacetic acid (TFA) to a final TFA concentration of 0.2% (v/v)

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Katerji M, Filippova M, Duerksen-Hughes P (2019) Approaches and methods to measure oxidative stress in clinical samples: research applications in the cancer field