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In lipid synthesis, fatty acids initially react with glycerol-3-phosphate to produce 1-acylglycerol-3-phosphate (LPA) in a reaction catalyzed by glycerol-3-phosphate acyltransferase (GPAT)

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ZO-1 is expressed in all cellular compositions after 7 days but the expression is changed after 28 days cultivation time

One-way ANOVA or the Students t -test was used for comparing conditions