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Inputs include influent dynamics, regulatory targets (DPR pathogen LRV, O 3 /BAC + RO + AOP barriers), unit models (kinetics, mass transfer, fouling, gas-liquid equilibria for CH 4 ), compatibilities and antagonisms, energy/GHG/cost models and control constraints

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Their use, however, is based on empirical evidence

Interestingly, occludin knockout mice had morphologically intact tight junctions but displayed complex histological phenotypes, with chronic inflammation and a defective epithelial barrier, which implicated that its crucial role was in tight junction stability rather than tight junction assembly

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