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Please read Part I - Mercury and Part II - Introduction to Detox before this article

10.1038/35067088 Nat Rev Mol Cell Biol

The supernatant was used for GSH and GSSG estimation

For the cell cycle assays, the Cell Cycle and Apoptosis Analysis Kit was used, and the cells were resuspended, fixed with cold 70% ethanol overnight, washed twice with PBS, and then incubated with propidium iodide for 30 min at 37C