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In general, antioxidants are essential for neutralizing and controlling the presence of harmful molecules in the body called free radicals

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This further supports why a higher drug concentration is needed at 48 h to achieve the same cytotoxic effect, considering 4T1 cells short doubling time of 13.6 h (Simoes et al

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Draw the calculated amount of bacteriostatic water with a mixing syringe, then inject it slowly down the inside wall of the peptide vial