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For viability staining, cells were stained with Cell-ID cisplatin 198Pt (Standard BioTools) at a final concentration of 5 M for 5 min at RT

Notably, the three-dimensional structure indicates that OsDHAR contains a broad, uncharged cleft between the 2-helix and the 3-helix as the putative binding site for non-native protein folding intermediates for its chaperone-like activity