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They found that glabridin upregulated the expression of LC3-II and Beclin1 and induced autophagy by modulating the phosphorylation of p38MAPK and JNK1/2, which subsequently led to cell death (100)

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We also observed an increase in the 18 kDa translocator protein TSPO, which is a mitochondrial cholesterol-binding protein and upregulated in microglia during inflammatory activation associated with neurodegeneration (Fig

Regarding liver and kidney damage in MTX treatment, Bedoui stated that MTX hepatotoxicity may occur due to the depletion of folate reserves and MTX-polyglutamate formation in the liver (Reference Bedoui, Guillot and Slambarom41)