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Instead, it has been hypothesized that Cys35 reacts with formaldehyde directly to form an S -hydroxymethyl adduct and, in the presence of a neighboring primary amine, a thiazolidine-like adduct (see Figure 1B

Most cyanocobalamin is provided at a 1000 microgram/milliliter concentration, so this often equates to 0.25ml

doi: 10.1128/mSystems.00901-19 59

A) Ascorbic acid (AA) can lose an electron to become monodehydroascorbate (MDHA) radical, which can lose an electron to become dehydroascorbate (DHA)

*Correspondence: Santu Ghosh, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

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