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10.2174/1567205012666150713102326 59 LiuY.DengY.LiuH.YinC.LiX.GongQ

73 Kennedy DO, Scholey AB, Wesnes KA

Twenty grams of whey protein isolate has been shown to maximally stimulate muscle protein synthesis. Thirty grams, 2x/day was shown to stimulate muscle protein synthesis significantly better than collagenwith and without resistance exercise. Twenty grams of whey protein taken after exercise significantly increased skeletal muscle mass, grip strength, and gait speed in healthy older women with sarcopenia. When combined with resistance exercise, whey protein significantly increases lean body mass. Supplementing with 20 grams of whey protein, especially when combined with and consumed after resistance training, can significantly improve muscle protein synthesis and lean muscle mass

In fact, ZIP7 also plays a crucial role in intestinal epithelial self-renewal

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We then expressed these proteins in HEK293T cells to determine their effect on autophagy by detecting the protein levels of SQSTM1/p62 (a substrate receptor degraded by autophagy) and LC3-II (autophagosome membrane marker protein degraded through autophagy)