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Both upregulated and downregulated proteins were computed in accordance with each network to conduct a pathway analysis and identify proteins associated with the pathways of interest ( We additionally used the hypothesis strategy GeneMANIA (freely available at to generate hypotheses regarding gene functions, calculate gene lists, and weight genes, the algorithm of which is designed to automatically weight networks based on the relevance to the gene set

Machine-learning approaches have demonstrated the capacity to analyze high-dimensional datasets linking NP physicochemical attributessuch as size, surface charge, lipid composition, ligand density, hydrophobicity, and structural topologywith their biological behavior in vivo, including hepatic tropism, immune recognition, complement activation, and endosomal escape efficiency [215,216,217,218]

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