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For the analysis of postischemic leukocyte responses, three postcapillary vessel segments in a central area of the spread-out cremaster muscle were randomly chosen

These compounds protect the intestinal barrier through their anti-inflammatory effects and by promoting the expression of TJs

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Then, the membranes were washed with phosphate-buffered saline with Tween-20 three times before incubated with the appropriate horseradish peroxidase-conjugated secondary antibody