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Tetrahydrofolate is further converted into 5,10-methylene-tetrahydrofolate (5,10-CH 2 -THF), which is subsequently reduced to 5-methyltetrahydrofolate (5-CH 3 -THF) (Preynat et al., 2009)

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While advanced methodologies like high-throughput sequencing, meta-omics approaches, and in vitro / in vivo models have deepened our understanding of ENMmicrobiome interactions, challenges persist in standardizing protocols and assessing long-term risks

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