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10.3389/fbioe.2022.895069

In this work, 25 L GSH (2.5 mM) and 45 L CDNB (3.0 mM) were used as the substrate of the enzyme reaction, and pH 6.5 TrisHCl (20 mM) buffer was used as the enzyme reaction media

3.1.1 Enzyme-responsive nanomaterials Enzyme-responsive stimulus is an ideal choice for their application in biomedical field due to several advantages like high sensitivity and selectivity, catalytic efficacy, biorecognition, mild reaction conditions, and easy decomposition [49, 50]