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One possible explanation for such a response is that inhibition of mTORC1 and activation of GCN2 collectively suppresses global translation to conserve nutrients, while selective translation of mRNA such as ATF4 (mediated by increased p-eIF2) activates the expression of AARE genes to mitigate cellular stress

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Then, the lysate was centrifuged at 4 C, 17,900 g for 5 min, and the supernatant was collected