Tissues were obtained through the National Disease Research Interchange (Philadelphia, PA) with approval from the University of Pittsburgh Committee for Oversight of Research and Clinical Training Involving Decedents (CORID)

Complete Blood Count / CBC (includes Differential and Platelets): WBC, RBC, Hemoglobin, Hematocrit, MCV, MCH, MCHC, RDW, Platelet Count, MPV and Differential (Absolute and Percent - Neutrophils, Lymphocytes, Monocytes, Eosinophils, and Basophils) Comprehensive Metabolic Profile ( includes eGFR ): Albumin, Albumin/Globulin Ratio (calculated), Alkaline Phosphatase, ALT, AST, BUN/Creatinine Ratio (calculated), Calcium, Carbon Dioxide, Chloride, Creatinine with GFR Estimated, Globulin (calculated), Glucose, Potassium, Sodium, Total Bilirubin, Total Protein, Urea Nitrogen C-Reactive Protein (CRP), High Sensitivity (Cardiac Risk Assessment) Dehydroepiandrosterone (DHEA-s) Sulfate Estradiol, Ultrasensitive, LC/MS/MS Hemoglobin (Hgb) A1c Homocysteine, Cardiovascular Lipid Panel: Cholesterol, total
Some patients notice improvements within a few weeks, while others may require a few months to experience significant benefits
Seasonal considerations matter too
Increased expression of Braf, Egfr, Egr1, Hdac7, Mapk1, Mapk3, Mapk14, Plcg1, Prkcg, Ptk2, Pxn, Src, Srf, and Vegfa is noted at 5 min
Forcing a reconstituted peptide solution through an extremely narrow needle (a very high gauge) with too much pressure can potentially shear the delicate protein chains