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The amide backbones of the mutant Ala253 residues in adjacent activation loops are separated by 17 , and both of the alanine side chains face inward, toward the center of the tetramer

This suggests that hepatotoxicity will occur if the patient is not treated properly (normal serum half-life = 2.02.5 hours

Curr Drug Metab 21(4):260269 Nielsen SE, Breinholt V, Justesen U, Cornett C, Dragsted LO (1998) In vitro biotransformation of flavonoids by rat liver microsomes

You can see the location of this nerve in the following image

Pol Merkur Lekarski 48(284):124127 Alkadi H (2020) A review on free radicals and antioxidants