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Lee) polysaccharides promote the longevity of Caenorhabditis elegans through autophagy-dependent insulin and mitochondrial pathways

First the vector was digested in a reaction with Fast Digest Buffer (B64, Thermo Fisher Scientific), BsmBI restriction enzyme (FD0454, Thermo Fisher Scientific), DTT (10 mM) with program 45 min at 37 C, heat inactivate 10 min at 65 C

[DOI] [PubMed] [Google Scholar] 18.Paliwal R., Sharma V., Pracheta P

This occurs via the lipid metabolism hub gene Phospholipase A2 Group IIA in homoeostatic chondrocytes, a Phospholipase A2 enzyme that promotes OA pathology by increasing arachidonic acid release and activating downstream pro-inflammatory pathways (Wang Y