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Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations

The amount of DON in each sample was determined using a high-performance liquid chromatography-mass spectrometer/mass spectrometer (HPLC-MS/MS) system (a Shimadzu 30A LC system coupled to a Triple Quad 6500+, Sciex, United States)

Dec 2015;11(3):202-6

Research Use Cases: Research shows that Bacteriostatic Water is primarily used for the reconstitution of peptides and other injectables in clinical and laboratory settings

If applied locally via injection, as was done in the study, there is also a risk of structural damage as the needle could break the fragile external walls that protect the discs

If you accidentally added too little water, you can add more bacteriostatic water to reach your intended total volume