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doi:10.1186/s41043-015-0032-y [28] Woodford KB

While some evidence supports NAC use to prevent contrast-induced nephropathy, NAC otherwise has not been consistently shown to improve perioperative renal outcomes, the most-studied perioperative indication

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Subsequently, gentamicin (300 g/mL) was added for 15 min, followed by PBS washes

The film was hydrated for 1 h using phosphate buffer pH 7.4, and the dispersion was sonicated using a probe sonicator (PRO650, LABMAN, Chennai, Tamil Nadu, India) at a reduced temperature (using ice bath) to decrease the size of the nanovesicles

Within 90 minutes of ingestion, cellular glutathione levels were seen to substantially increase reaching maximum levels after two to three hours, before returning to basal levels by five to 6 hours