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The aberrant accumulation of A and hyperphosphorylated Tau proteins on mitochondria results in mitochondrial respiratory chain complex I activity, impaired mitochondrial membrane potential (MMP), and massive production of reactive oxygen species (ROS), which in turn affects mitochondrial function ( A number of studies have demonstrated that there are extensive mitochondrial abnormalities in the brains of AD patients and animal models (Swerdlow, 2018)

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Vitamin B12 is available in oral supplements the highest doses comes from those supplements which only contain vitamin B12 rather than medicines which combine it with other B-complex vitamins or other minerals and supplements (e.g

ab6326, Abcam, Cambridge, UK) overnight at 4 C

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Skin condition was assessed through by macroscopic observation and histological examination