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Other Uses: Rotational use in GH optimization programs to support consistent results while reducing the risk of receptor desensitization

Afterward, the samples were washed 3 times in PBS-T [0.2% (v/v) Tween 20 in PBS] and incubated with secondary antibodies: Cy3-conjugated donkey anti-rat secondary antibody (ImmunoResearch Europe, 712-166-153

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The protocol does not require a specific time of day

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