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Fractions with a purity of greater than 95% were pooled and lyophilized

In experiments in which large amounts of acetyl-CoA were incubated with purified enzyme to test its protection against SNO-CoA-mediated inhibition, varying levels of acetyl-CoA were added back into the assay solutions after the incubation period, such that in the assay condition, the concentration of acetyl-CoA was even across samples to control for the possible effect of acetyl-CoA as a competitive inhibitor of PDHC

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This might reflect an adaptation to favor the maintenance of NADPH pools, an essential co-factor in hundreds of biochemical reactions, at the expense of other critical pathways including energy production and nucleotide-activated sugar synthesis

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