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After several wash cycles, the tumor sections were blocked and incubated with an anti-human MDM2 antibody and pre-diluted streptavidinperoxidase horseradish peroxidase conjugates, using a staining kit from Dako North America Inc

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Western blotting assay was used to detect whether AP probe-net alters the expression levels of proapoptotic proteins in THJ-16T and CAL-62 cells

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