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Cells were washed, colleted with 1 mM EDTA in PBS, pelleted (500 g for 5 min) and re-suspended in isolation medium (320 mM sucrose, 10 mM TrisHCl, 1 mM EDTA, pH 7.4)

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In thyroid diseases, metabolic disorders associated with low-grade inflammation can also lead to an increased oxidative stress (68)

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