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These ions are asymmetrically distributed between luminal and abluminal membranes, of which efflux and influx were mainly dependent on the ion transporters on BBB

This design minimises bias and provides the most reliable evidence of treatment efficacy

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Given the results of MTT assay and Ki-67 staining (Supplementary Figure 2) suggesting an optimal intensity, the human dermal papilla cells were exposed with or without NTAPP (4.2 kV) for 1 min, and were further incubated for 24 h