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The resin was washed once with His lysis buffer and three times with His 6 wash buffer (50 mM Tris-HCl pH 7.5, 300 mM NaCl, 20 mM imidazole, 5% glycerol, 2.5 mM ME)

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As shown in Table 3, the measured color parameters L* (lightness), a* (redgreen axis), and b* (yellowblue axis) differed among samples K, A, B, and C