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When evaluating the binding energy of isoxazole 1 using this method, we found a much smaller effect (8.3 kcal/mol for Cn Acs1 vs 8.1 kcal/mol for Cn Ca Acs1), but one that still showed reduced preference for the Ca Acs2 binding site residues

doi: 10.3389/fimmu.2023.1137089

They were discovered by a Dr William Hoekstra of the University of Wisconsin in 1973 [10]