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During purification, the H2B-Npu N was loaded on the column in 2 M urea and incubated at pH 6.0 for 3 h, followed by a change to pH 7.2 to optimize the thiolysis reaction in the presence of MES (36 h at room temperature)

doi: 10.1111/bdi.13049 250 HasegawaSGotoSTsujiHOkunoTAsaharaTNomotoKet al

Li Y, Liu C, Yang L, Li L, Hong L

In this experiment, they exposed U937 lymphoma cells pretreated with IF to high dose ionizing radiation

Meanwhile, during the ferroptosis, cells release oxygenated phosphatidylethanolamines on the outer plasma membrane, which are detected by macrophages via toll-like receptor 2, allowing the clearance of ferroptosis cells (76)

Its often discovered incidentally during a routine colonoscopy