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The cultured OLs were homogenized using RIPA lysis buffer containing protease and phosphatase inhibitors, followed by centrifugation at 4 C

10.3390/ijms222011264 39 HanaokaY.YamaguchiY.YamamotoH.IshiiM.NagaseT.KuriharaH.et al

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The present study revealed that the levels of LPO and H 2 O 2 in the testes significantly increased when APAP and alcohol were administered [Figure 4a and b]