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Supernatants were diluted with ChIP dilution buffer (50 mM Tris-HCl [pH 8.0], 167 mM NaCl, 1.1% Triton X-100, 0.11% NaDoc, 200 nM PMSF), precleared with normal rabbit IgG (Cell Signaling Technology, #2729) or normal mouse IgG (Santa Cruz, sc-2025), and incubated with Protein G agarose beads (Thermo Scientific, 20398) pre-washed in 3.125 mg/mL BSA/ChIP dilution buffer for 2 h at 4 C

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Stromal changes in the aged lung induce an emergence from melanoma dormancy

For example, DNA demethylation is important for primordial germ cells (PGCs) to gain pluripotent ability

The half-life is 78 h