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[DOI] [PubMed] [Google Scholar] 6.Vigliani E.C., Baldi G

Determination of free iron content Senescent A549 cells were seeded in T75 cell culture flasks (2 10^6 cells per flask) and cultured for 24 h, and the nano-drugs were added to the corresponding cell cultures for another 4 h

Administration of PLX3397 to mice For pharmacological ablation of microglia, PLX3397 was administrated to mice by supplementation (290 mg/kg of PLX3397 (#C-1271

In addition, a GSH/GSSG assay kit was used in this study, which is capable of accurately quantifying GSH/GSSG in cells by utilizing the enzymatic reaction between 5, 5-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase (GR)

AAs are derived from tyramine and are unique to the Amaryllidoideae family, where they contribute to plant defense under abiotic and biotic stress (Liyanage et al

When ROS increases, the synthesis of mitochondrial fusion-related proteins will be inhibited, thus promoting mitochondrial fission, which in turn may lead to more ROS production and severe mitochondrial dysfunction