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2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

E9 cells were seeded at 7000 cells per well in a 96-well plate, and after attachment, treated with ZNT for 24 h at 37 C and 5% CO 2

TRLs are a family of pattern recognition receptors that bind to endogenous ligands released by damaged cells (damage-associated molecular patterns, DAMPs), which can be released in large quantities by the mass apoptotic islets early after transplantation (28)

Butyrate, a SCFA derived from probiotic metabolism, downregulates the transcription factor RORt in Th17 cells, curtailing IL-17A secretion a key driver of lupus nephritis considerably lowering pro-inflammatory cytokines like TNF- and IL-6 (146) ( Table 2 )