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Metabolomics: Official journal of the Metabolomic Society 6, 7895, 10.1007/s11306-009-0178-y (2010)

Plasmids originated from the pFL44L-based library were extracted and purified with the Zymoprep kit (Zymo Research), amplified in Escherichia coli and then retransformed into the yeast strain overexpressing CYS4 to confirm their ability to reverse methionine auxotrophy

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A fluorometric assay for measurement of PPO activity in red blood cell lysates has been developed (Bates and Powers, 1985) and subsequently optimized and adapted for assessment of PPO AC (Mushtaq et al., 2009)