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Influenza infection induces host DNA damage and dynamic DNA damage responses during tissue regeneration

10.1097/MPG.0000000000002185 119

Immunohistochemistry Formalin-fixed, paraffin-embedded tissue sections (5 m) were used for haematoxylin and eosin staining and immunohistochemical analyses

Possible Cause: Complexes not thoroughly mixed in growth medium Suggested Solution: Following addition of transfection complexes into medium, ensure that the plate or wells are thoroughly mixed to prevent concentration of DNA:transfection reagent complexes in the wells Possible Cause: Cells have changed over time, or splitting conditions have changed Suggested Solution: If transfection performance suddenly declines, it may be because of the cells

their export across the plasma membrane is entirely dependent on the mammalian transporter ferroportin-1 (FPN1) (Nemeth and Ganz, 2021)