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Mock-treated samples were supplemented with an equivalent volume of DMSO

Nevertheless, a subset of patients continues to face therapeutic challenges associated with primary/secondary treatment failure, adverse reactions, and disease heterogeneity, which has driven the development of emerging therapies based on pathological mechanisms

The samples of blood/liquor were collected in rated intervals (see experimental design) from the jugular vein or cisterna magna respectively, via the polyethylene tubing (BTPE10, Instech, USA) applied before the glutamate application

At the molecular level, zinc deficiency downregulates TJ proteins such as claudin-3 and occludin, leading to increased permeability and compromised barrier integrity (Zhong et al., 2010)

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