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Antibiotic medium (MEM, 2% PS, 12.5 g/mL amphotericin B, 0.5 mg/mL gentamicin sulfate) was added and the cells were cultured in an incubator (28C + 5% CO2) for 2 hours, with a medium change every 30 minutes

A diploid, heterozygous yeast strain was constructed in which URA3 was expressed as counter-selectable marker in the BY4743 background

[PubMed: 21730278] 127

The phenylpropane biosynthesis pathway is considered to play an important role in a plants response to abiotic stress

The use of MYCN-regulatable cell systems only moderately affected treatment sensitivity

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