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There was no significant main effect of infusion block ( F 5,253 = 0.33, P = 0.896) or condition-by-block interaction ( F 5,253 = 1.29, P = 0.270)

Single-cell suspensions were then loaded onto microfluidic devices, and single-cell RNA sequencing (scRNA-seq) libraries were constructed according to the Singleron GEXSCOPE protocol via the GEXSCOPE Single-Cell RNA Library Kit (Singleron), which included cell disruption, mRNA trapping, cell labeling (with barcodes) and mRNA labeling (with unique molecular identifier (UMI)), reverse transcription of mRNA into complementary DNA (cDNA) and amplification, cDNA fragmentation, and finally cDNA fragment cloning

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This doesn't necessarily mean that all vegetarians need B12 injections, but it is advisable for vegans and vegetarians be mindful of their B12 intake to ensure they are getting sufficient levels of this vital nutrient